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   *ø  qGACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,GCACCCANNNGTT,AACNNNTGGGTGC,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,CACCCA,TCGGTC,TGGGTG,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                            "                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
      	                        	                                                                                                                 	                                                                                                                                  "                	                                                                     
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      	                        ,      	                
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         	                           >            &   
      %         f   1   	                  8                                                                                                                                                                       "                                                                                                                                                                                                                                                                                           
  	          	                                               	                                                  "      	                           
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  	        ,  	      
         	    	       
   	         >    & 
  %   f 1 	      8   \,  ]@  ]b  ]�  `º  dº  i”#
\y‡gy9�·Ø	Í	ß	…
œŽó(;� ¸î Ñé| ÌtX ÝMÇÈ…	
'¦ø{�,ð£ë€€YeŠÖ[ü‡g çmÖQ ¦4nÊø%W{„è[d	u	~	³	¼	ì	(�«
�],ðüÛ‹&	Ø¾	ŒºØ	Ø	ŒºØØ	éZ	½Ôu¼uØ	•�¼p¥ïŽeŠ,Ž	•‘™WF‘å•ßð3<™	0F Q H‚K¬Þ™ò]	$	!	oG éV‘ïoxPB —7ú"5H‡š­[õxöJu¹	%Ék§K'	B*=�¢dkAY›æn—²9¼ü ²ú«¶ÌxPõx¦BÉ_n? Å1@ Ó÷
÷
 	å÷K÷
›n9Ñ‹	j
õxÉŠ
Û#øõŽ
F{gU¤z Æ
…XÚ õxÊ}	±
ÉÎÔèœ±[¿õOxŠ•öJu	¹	Û	
%�ÉoÐ®-'
B áS»~¡ª6VWù†·Øò´	]
Ù”šNÐ—‹
"1†+m€Æ1í“ºÈæ—ìÄóí
æ£	—^¨~ÙåÑ‹Ó÷K÷
	o�>Åá~†Ù¿Zâ )ÈÛ#8W�íùm5	R	Ì	ñ	!pËÏøüJÆ;?“õùThlË~6ùÙÅÛ#øõÔ’>IŸ«èÞ}³Ì1	ù²Å5Ncmx
—
Fˆ®Ð®ÌÆU&ÚõøÉ¾ü Ý%ò ; òn
|ö÷
ß$ç¼—B	¦
,ŽðC�|3<á>©º{
3þÆe tùT	j .Cè«- 	z Xe T	j 	'
-BŸã“>(ß¢	o-"5H‡š­  WÎÑó—Ò$º’>Ÿ	•ôJ¬D†@ßÌT¼óWu™·^´	Ø	Q
^
F”š©;Âd µ`ÈŒV	›	TW%÷úZ²Àæ5åZš7ƒT	%	"I‘V  SÙòr8Žçê£<Kg„®4:¥ÖàšT‘d `ÈŒTW%÷úZ²µV	›	Àæ5�.SñþWfuhiÐó£–±»ß\Íðd *v¦knµø[`{Èå)ŒV	›	é	„
TW%(Q™¨÷ú#MZ§²Àæ5ùSbq  ÌT¼óWSu™·Ùò^r´	Ø	Q
^
8FŽ”çêšð…ß'«
Ó«ª®Ï­iP]Ûâh>	ÒV¥¿	yñTÿò1:¬TÿÐ
iDn˜³v£¸ @SgŽ8� 6Þ	´í= 3F…˜«G R I¡³Ð
iDn˜ÜãkÀÎhfÌ1	’	O\¥#
e£H_nÌ§xBWH€9Žúj6	Kò©T’ \¦`–	0ÔÄB záLO‘Íñ¢ÏAD"
¢¬¹Éë ©Ð²ÿ-
{
ÜbéEo™­':MŒŸ²ÞIþ(	£
ÙBVr~™A¾XmQEèìŸ ƒ¤=L­9™"aÕß”¨â ÓŠ¯G-ÍÀ¢[ë-[0¦–	ÔÄƒ¤=L­9ß¨’ \`*v¦knø[{å)é	„
(Q™¨#M§ùSbqÎÜãhfÌ1	’	O\kÀ„^¾Šý/R @‚*~¿_z|†²Jp‡ÉËëú;]�îXë	0	]J”t°Ç+6y…±COXdny§Ilo��ù:\|�û=Wk7	…	ê	
…
˜
)GR_‰š©L¯	!$Nk¥¨ósúTcrv©ìû%Må)	DT,?‘¤bÊp._Z#6Iˆ›®P f o LmG¤¶ì´Ž	U	i	d
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   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  Ç<?xml version="1.0"?><Features nextValidID="11"><Feature recentID="0" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4112-4216" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="NeoR/KanR" directionality="2" translationMW="28979.67" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="3317-4111" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="2" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1575-2174" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2555-3143" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4559-4577" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="hsp70 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-239" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;Drosophila melanogaster&lt;/i&gt;&amp;nbsp;hsp70Bb promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1186-1515" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2177-2298" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1051-1162" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="Factor Xa site" directionality="1" translationMW="473.54" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4593" consecutiveTranslationNumbering="1" maxRunOn="4812" maxFusedRunOn="4812" detectionMode="exactProteinMatch"><Segment range="4582-4593" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="10" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4532-4548" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  ~<?xml version="1.0"?><Primers nextValidID="13"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="BGH-rev" sequence="TAGAAGGCACAGTCGAGG" description="Bovine growth hormone terminator, reverse primer. Also called BGH reverse"><BindingSite location="1044-1061" boundStrand="1" annealedBases="TAGAAGGCACAGTCGAGG" meltingTemperature="55"><Component hybridizedRange="1044-1061" bases="TAGAAGGCACAGTCGAGG"/></BindingSite><BindingSite simplified="1" location="1044-1061" boundStrand="1" annealedBases="TAGAAGGCACAGTCGAGG" meltingTemperature="55"><Component hybridizedRange="1044-1061" bases="TAGAAGGCACAGTCGAGG"/></BindingSite></Primer><Primer recentID="1" name="EBV-rev" sequence="GTGGTTTGTCCAAACTCATC" description="SV40 polyA terminator, reverse primer"><BindingSite location="2267-2286" boundStrand="0" annealedBases="GTGGTTTGTCCAAACTCATC" meltingTemperature="55"><Component hybridizedRange="2267-2286" bases="GTGGTTTGTCCAAACTCATC"/></BindingSite><BindingSite simplified="1" location="2267-2286" boundStrand="0" annealedBases="GTGGTTTGTCCAAACTCATC" meltingTemperature="55"><Component hybridizedRange="2267-2286" bases="GTGGTTTGTCCAAACTCATC"/></BindingSite></Primer><Primer recentID="2" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="2444-2461" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="2444-2461" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="2444-2461" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="2444-2461" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="3" name="M13 Forward" sequence="TGTAAAACGACGGCCAGT" description="In lacZ gene. Also called M13-F20 or M13 (-21) Forward"><BindingSite location="4530-4547" boundStrand="0" annealedBases="TGTAAAACGACGGCCAGT" meltingTemperature="57"><Component hybridizedRange="4530-4547" bases="TGTAAAACGACGGCCAGT"/></BindingSite><BindingSite simplified="1" location="4530-4547" boundStrand="0" annealedBases="TGTAAAACGACGGCCAGT" meltingTemperature="57"><Component hybridizedRange="4530-4547" bases="TGTAAAACGACGGCCAGT"/></BindingSite></Primer><Primer recentID="4" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="4516-4538" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="4516-4538" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="4516-4538" boundStrand="0" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="4516-4538" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="5" name="pBABE 3'" sequence="ACCCTAACTGACACACATTCC" description="SV40 enhancer, reverse primer for pBABE vectors"><BindingSite location="1180-1200" boundStrand="1" annealedBases="ACCCTAACTGACACACATTCC" meltingTemperature="56"><Component hybridizedRange="1180-1200" bases="ACCCTAACTGACACACATTCC"/></BindingSite><BindingSite simplified="1" location="1180-1200" boundStrand="1" annealedBases="ACCCTAACTGACACACATTCC" meltingTemperature="56"><Component hybridizedRange="1180-1200" bases="ACCCTAACTGACACACATTCC"/></BindingSite></Primer><Primer recentID="6" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="2634-2653" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="2634-2653" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="2634-2653" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="2634-2653" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="7" name="pCasper-hs" sequence="GCAACTACTGAAATCTGCCAAG" description="Drosophila Hsp70 promoter, forward primer"><BindingSite location="185-206" boundStrand="0" annealedBases="GCAACTACTGAAATCTGCCAAG" meltingTemperature="57"><Component hybridizedRange="185-206" bases="GCAACTACTGAAATCTGCCAAG"/></BindingSite><BindingSite simplified="1" location="185-206" boundStrand="0" annealedBases="GCAACTACTGAAATCTGCCAAG" meltingTemperature="57"><Component hybridizedRange="185-206" bases="GCAACTACTGAAATCTGCCAAG"/></BindingSite></Primer><Primer recentID="8" name="Puro-F" sequence="GCAACCTCCCCTTCTACGAGC" description="Puromycin resistance gene, forward primer"><BindingSite location="2070-2090" boundStrand="0" annealedBases="GCAACCTCCCCTTCTACGAGC" meltingTemperature="60"><Component hybridizedRange="2070-2090" bases="GCAACCTCCCCTTCTACGAGC"/></BindingSite><BindingSite simplified="1" location="2070-2090" boundStrand="0" annealedBases="GCAACCTCCCCTTCTACGAGC" meltingTemperature="60"><Component hybridizedRange="2070-2090" bases="GCAACCTCCCCTTCTACGAGC"/></BindingSite></Primer><Primer recentID="9" name="Puro-R" sequence="GTGGGCTTGTACTCGGTCAT" description="Puromycin resistance gene, reverse primer. Also called puro-variant-R"><BindingSite location="1574-1593" boundStrand="1" annealedBases="GTGGGCTTGTACTCGGTCAT" meltingTemperature="59"><Component hybridizedRange="1574-1593" bases="GTGGGCTTGTACTCGGTCAT"/></BindingSite><BindingSite simplified="1" location="1574-1593" boundStrand="1" annealedBases="GTGGGCTTGTACTCGGTCAT" meltingTemperature="59"><Component hybridizedRange="1574-1593" bases="GTGGGCTTGTACTCGGTCAT"/></BindingSite></Primer><Primer recentID="10" name="SV40pA-R" sequence="GAAATTTGTGATGCTATTGC" description="SV40 polyA, reverse primer"><BindingSite location="2213-2232" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="52"><Component hybridizedRange="2213-2232" bases="GAAATTTGTGATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="2213-2232" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="52"><Component hybridizedRange="2213-2232" bases="GAAATTTGTGATGCTATTGC"/></BindingSite></Primer><Primer recentID="11" name="SV40pro-F" sequence="TATTTATGCAGAGGCCGAGG" description="SV40 promoter/origin, forward primer"><BindingSite location="1427-1446" boundStrand="0" annealedBases="TATTTATGCAGAGGCCGAGG" meltingTemperature="57"><Component hybridizedRange="1427-1446" bases="TATTTATGCAGAGGCCGAGG"/></BindingSite><BindingSite location="1450-1461" boundStrand="1" annealedBases="CAGAGGCCGAGG" meltingTemperature="51"><Component bases="TATTTATG"/><Component hybridizedRange="1450-1461" bases="CAGAGGCCGAGG"/></BindingSite><BindingSite simplified="1" location="1427-1446" boundStrand="0" annealedBases="TATTTATGCAGAGGCCGAGG" meltingTemperature="57"><Component hybridizedRange="1427-1446" bases="TATTTATGCAGAGGCCGAGG"/></BindingSite><BindingSite simplified="1" location="1450-1461" boundStrand="1" annealedBases="CAGAGGCCGAGG" meltingTemperature="51"><Component bases="TATTTATG"/><Component hybridizedRange="1450-1461" bases="CAGAGGCCGAGG"/></BindingSite></Primer><Primer recentID="12" name="T7" sequence="TAATACGACTCACTATAGGG" description="T7 promoter, forward primer"><BindingSite location="4558-4577" boundStrand="0" annealedBases="TAATACGACTCACTATAGGG" meltingTemperature="50"><Component hybridizedRange="4558-4577" bases="TAATACGACTCACTATAGGG"/></BindingSite><BindingSite simplified="1" location="4558-4577" boundStrand="0" annealedBases="TAATACGACTCACTATAGGG" meltingTemperature="50"><Component hybridizedRange="4558-4577" bases="TAATACGACTCACTATAGGG"/></BindingSite></Primer></Primers>
  ¼<Notes>
<UUID>eabccf40-699b-4df1-898f-863511938f7a</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pBait-YY1</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>Bait plasmid for PROBER with YY1-binding site (positive control).</Description>
<Created UTC="14:56:33">2021.7.15</Created>
<LastModified UTC="14:56:33">2021.7.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference journalName="Unpublished" type="Journal Article" title="PROBER Identifies Proteins Associated with Sequence-Specific DNA in Living Cells" journal="Unpublished"/>
</References>
</Notes>
  Y     –         
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   )<?xml version="1.0"?><CustomEnzymeSets/>
